Identificación genética del orégano de Putre (Origanum vulgare L) mediante ITS y microsatélites, una especie reconocida en Chile con Sello de Origen / Genetic identification of the Putre's oregano (Origanum vulgare L) by ITS and microsatellites, a species recognized in Chile with Seal of Origin

Putre ́s oregano (Origanum vulgare L.) is a variety of oregano that grown in the Arica-Parinacota Region. Its organoleptic attributes and unique production conditions have earned it a certification with Geographical Indication (GI). However, the demands of the markets require a scientific-technological support for identification and authentication of materials. In this context, was proposed to identify Putre's oregano by phylogenetic relationships based on the use of molecular markers SSR and "DNA Barcode". The results showed that when comparing materials from different sources of Putre ́s oregano versus information from certified germplasms and GenBank sequences, added to the analysis with nuclear genetic markers, Putre ́s oregano corresponds to the species Origanum vulgare L. subsp virens. This precise identification will support the correct differentiation and authentication of this genotype, serving in addition to supporting the GI.

El orégano de Putre (Origanum vulgare L.) es una variedad de orégano que se cultiva en la Región de Arica y Parinacota. Sus atributos organolépticos y condiciones únicas de producción lo han hecho acreedor de una certificación con Indicación Geográfica (IG). Sin embargo, las exigencias de los mercados requieren de un respaldo científico-tecnológico de identificación y autenticación de materiales. En este contexto, se propuso identificar el orégano de Putre mediante relaciones filogenéticas a partir del uso de marcadores moleculares SSR y "DNA Barcode". Los resultados demostraron que al comparar los materiales de distintas procedencias de orégano de Putre versus la información desde germoplasmas certificados y secuencias de GenBank, sumado al análisis con marcadores genéticos nucleares, el orégano de Putre corresponde a la especie Origanum vulgare L. subsp virens. Esta identificación precisa dará soporte a la correcta diferenciación y autenticación de este genotipo, sirviendo además de apoyo a la IG.

Effect of biofertilization and organic manuring on soil dehydrogenase activity, macronutrients and essential oil content of marjoram

Two field experiments were carried out at an organic farm [Minoufia Governorate] during 2005- 2006 and 2006-2007 seasons to study the interaction effect of biofertilization and organic manuring on dehydrogenase, polyphenol oxidase and peroxidase activity, macronutrients uptake, oil yield and oil composition of marjoram. Greater activities of dehydrogenase, peroxidase and polyphenol oxidase were obtained in soil and marjoram treated with combination of biofertilizers and organic manure than soil treated with biofertilizers or organic manure. The greatest activities of peroxidase and polyphenol oxidase were obtained with the treatment of biofertilization combined with full dose of organic manure. Similar results trend was observed with N, P and K uptake because N, P and K uptake by marjoram were greater with dual application of biofertilization and organic manure than other treatments. There is no significant difference between oil yield obtained with chemical fertilization treatment and biofertilizers combined with full dose of organic manure treatment. Fourteen compounds, accounting for more than 97% of the total volatiles components in most marjoram samples were detected and identified. Dual application of organic manure and biofertilization to soil and marjoram produced maximum percent of terpincn-4-ol [the major compound in marjoram oil] compound in marjoram